Bile Acid Analysis – Synthesis and Metabolism Assay
Quantitative Metabolomics

Absolute Quantitation

Quantification of unconjugated, taurine- and glycine-conjugated bile acids and bile acid synthesis intermediates by UPLC-MRM/MS.

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Price
Academic
$400 1st sample; $120 each additional sample
Industry
$800 1st sample; $240 each additional sample
Minimum Volume

Tissue: 10 mg

Cell: 2×10^6

Blood/plasma/serum: 20 µL

Urine: 10 µL

Plant: N/A

Feces: 5 mg

DBS: 20 µL

Instrument
UPLC-MS (UPLC-MRM/MS)
Chemical Class

bile acids,
bile acid synthesis intermediates

Metabolic Pathways

Bile acid synthesis and metabolism,
gut co-metabolism,
cholesterol metabolism,
blood-brain barrier

Compounds

 

Name HMDB ID Super Class Class
3α,7α-diOH cholestenoic acid (DHCA)      
3α,7α, 12α–triOH cholestenoic acid (THCA)    
3β-OH cholestenoic acid      
3β,7α-diOH cholestenoic acid      
7α-OH 3-oxo-4-cholestenoic acid      
Apocholic acid      
Chenodeoxycholic acid 24-β-D-glucuronide      
Chenodeoxycholic aicd-3-glucuronide      
Deoxycholic acid – 24- glucuronide      
Deoxycholic acid – 3- glucuronide      
Dioxolithocholic acid      
Glyco-ursocholanic acid      
Glycoallocholic acid (no STD, deduced)      
Glycocholic acid 3-sulfate      
ω-Muricholic acid      
λ-Muricholic acid (hyocholic acid)      
α-Muricholic acid      
β-Muricholic acid      
Isodeoxycholic acid      
Lithocholic acid-3-glucuronide      
Murocholic acid      
Tauro- β-muricholic acid      
Tauro-ω-muricholic acid      
Tauro-α-muricholic acid      
Tauro-ursocholanic acid      
Taurodeoxycholic acid 3-sulfate      
Taurohyocholic acid (Tauro-λ-muricholic acid)    
Tauroursodeoxycholic acid 3-sulfate      
12-Ketochenodeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
12-Ketolithocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
3-Oxocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
6,7-Diketolithocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
7-Ketodeoxycholic acid HMDB0000391 Lipids and lipid-like molecules Steroids and steroid derivatives
7-Ketolithocholic acid HMDB0000467 Lipids and lipid-like molecules Steroids and steroid derivatives
Allocholic acid HMDB0000505 Lipids and lipid-like molecules Steroids and steroid derivatives
Alloisolithocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Chenodeoxycholic acid HMDB0000518 Lipids and lipid-like molecules Steroids and steroid derivatives
Cholanic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Cholic acid HMDB0000619 Lipids and lipid-like molecules Steroids and steroid derivatives
Cholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Dehydrocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Dehydrolithocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Deoxycholic acid HMDB0000626 Lipids and lipid-like molecules Steroids and steroid derivatives
Deoxycholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycochenodeoxycholic acid HMDB0000637 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycochenodeoxycholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycocholic acid HMDB0032596 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycodehydrocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycodeoxycholic Acid HMDB0000631 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycodeoxycholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycohyocholic acid HMDB0240607 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycohyodeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycolithocholic acid HMDB0000698 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycolithocholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Glycoursodeoxycholic acid HMDB0000708 Lipids and lipid-like molecules Steroids and steroid derivatives
Hyodeoxycholic acid HMDB0000733 Lipids and lipid-like molecules Steroids and steroid derivatives
Isolithocholic acid HMDB0000717 Lipids and lipid-like molecules Steroids and steroid derivatives
Lithocholic acid HMDB0000761 Lipids and lipid-like molecules Steroids and steroid derivatives
Lithocholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Lithocholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Lithocholic acid-24-glucuronide   Lipids and lipid-like molecules Steroids and steroid derivatives
Norcholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Nordeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Norursodeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurochenodeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurochenodeoxycholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurocholic Acid HMDB0000036 Lipids and lipid-like molecules Steroids and steroid derivatives
Taurodehydrocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurodeoxycholic Acid HMDB0000896 Lipids and lipid-like molecules Steroids and steroid derivatives
Taurohyodeoxycholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurolithocholic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Taurolithocholic acid 3-sulfate   Lipids and lipid-like molecules Steroids and steroid derivatives
Tauroursodeoxycholic Acid HMDB0000874 Lipids and lipid-like molecules Steroids and steroid derivatives
Ursocholanic acid   Lipids and lipid-like molecules Steroids and steroid derivatives
Ursocholic Acid HMDB0000917 Lipids and lipid-like molecules Steroids and steroid derivatives
Ursodeoxycholic acid HMDB0000348 Lipids and lipid-like molecules Steroids and steroid derivatives