Central Carbon Metabolism
Quantitative Metabolomics

Absolute Quantitation

Quantification of glucose, sugar phosphates, CoAs, enzyme co-factors, TCA cycle carboxylic acids, phosphocarboxylic acids and major nucleotides involved in Central Carbon Metabolism (CCM) and glucosamine metabolism by UPLC-MRM/MS

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Price
Academic
$400 1st sample; $180 each additional sample
Industry
$800 1st sample; $360 each additional sample
Minimum Volume

Tissue: 10 mg Cell: 2×10^6
Blood/plasma/serum: 100 µL
Urine: N/A
Plant: 50 mg
Feces: N/A
DBS: 50 µL
Cell medium: 100 uL

Instrument
UPLC-MRM/MS
Chemical Class

Sugars,
sugar phosphates,
nucleotides,
acetyl CoAs,
enzyme cofactors,
NAD(P)/NAD(P)H,
carboxylic acids,
phosphocarboxylic acids,
nucleotide sugars,
glucosamine phosphates,
galactosamine phosphates

Metabolic Pathways

Central carbon metabolism,
glucose metabolism,
hexose metabolism,
glucosamine metabolism,
TCA cycle,
glycolysis,
pentose phosphate pathway,
gluconeogenesis,
glyoxylate shunt,
GABA shunt,
shikimate pathway

Compounds

Name HMDB ID Super Class Class
6-Phosphogluconate (6PG)      
6-Phosphogluconone      
Deoxyribose 1-phosphate      
Dihydroxyacetone-P (DHAP)      
FAD      
FMN      
Fructose-1-P      
Fructose-1,6-bisP      
Glucosamine 6-P      
Glucose-1,6-bisP      
Glucose-6-P      
Glycerate-1,3-bisP      
Glycerate-2,3-bisP      
Glycerate-2P      
Glycerate-3P      
Glycerol-3P      
α-Hydroxyglutaric acid      
α-Ketogluteric acid      
β-Hydroxybutyric acid      
Mannose-6-P      
N-Acetyl-glucosamine 1-P      
Phosphoenolpyruvate (PEP)      
Ribose-1-P      
Ribose-1,5-bisP      
Ribulose-1,5-bisP      
Sedoheptulose-7P      
Shikimate-3P      
UDP-N-acetylglucosamine (UDP-GlcNAc)      
Xybulose-5P      
Acetoacetyl-CoA   Lipids and lipid-like molecules Fatty Acyls
Acetyl-CoA   Lipids and lipid-like molecules Fatty Acyls
Citraconic acid   Lipids and lipid-like molecules Lipids and lipid-like molecules
Citramalic acid HMDB0176421 Lipids and lipid-like molecules Fatty Acyls
Malonyl-CoA   Lipids and lipid-like molecules Fatty Acyls
Succinyl-CoA   Lipids and lipid-like molecules Fatty Acyls
ADP HMDB01341 Nucleosides, nucleotides, and analogues Purine nucleotides
ADP-glucose   Nucleosides, nucleotides, and analogues Purine nucleotides
AMP HMDB00045 Nucleosides, nucleotides, and analogues Purine nucleotides
ATP HMDB00538 Nucleosides, nucleotides, and analogues Purine nucleotides
cAMP   Nucleosides, nucleotides, and analogues Purine nucleotides
cGMP   Nucleosides, nucleotides, and analogues Purine nucleotides
GDP   Nucleosides, nucleotides, and analogues Purine nucleotides
GDP-fucose   Nucleosides, nucleotides, and analogues Purine nucleotides
GDP-glucose   Nucleosides, nucleotides, and analogues Purine nucleotides
GMP   Nucleosides, nucleotides, and analogues Purine nucleotides
GTP HMDB01273 Nucleosides, nucleotides, and analogues Purine nucleotides
HS-CoA   Nucleosides, nucleotides, and analogues Purine nucleotides
NAD+   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
NADH   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
NADP+   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
NADPH   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
UDP HMDB0062654 Nucleosides, nucleotides, and analogues Pyrimidine nucleotides
UDP-glucose HMDB00286 Nucleosides, nucleotides, and analogues Pyrimidine nucleotides
UMP HMDB00288 Nucleosides, nucleotides, and analogues Pyrimidine nucleotides
UTP   Nucleosides, nucleotides, and analogues Pyrimidine nucleotides
Acetoacetic acid HMDB0000060 Organic acids and derivatives Keto acids and derivatives
Acetoacetic acid HMDB0000060 Organic acids and derivatives Keto acids and derivatives
Aconitic acid HMDB0164614 Organic acids and derivatives Carboxylic acids and derivatives
Citric acid HMDB0000094 Organic acids and derivatives Carboxylic acids and derivatives
Fumaric acid HMDB00134 Organic acids and derivatives Carboxylic acids and derivatives
Glycolic acid HMDB0000115 Organic acids and derivatives Hydroxy acids and derivatives
Isocitric acid HMDB0000193 Organic acids and derivatives Carboxylic acids and derivatives
Lactic acid HMDB0041561 Organic acids and derivatives Hydroxy acids and derivatives
Malic acid HMDB0167333 Organic acids and derivatives Hydroxy acids and derivatives
Methylcitric acid   Organic acids and derivatives Carboxylic acids and derivatives
Methylcitric acid   Organic acids and derivatives Carboxylic acids and derivatives
Oxaloacetic acid HMDB00223 Organic acids and derivatives Keto acids and derivatives
Phosphocreatine   Organic acids and derivatives Carboxylic acids and derivatives
Pyruvic acid HMDB0141776 Organic acids and derivatives Keto acids and derivatives
Succinic acid HMDB0000254 Organic acids and derivatives Carboxylic acids and derivatives
Erythrose-4-P   Organic oxygen compounds Organooxygen compounds
Fructose-6-P   Organic oxygen compounds Organooxygen compounds
Glucaric acid   Organic oxygen compounds Organooxygen compounds
Gluconic acid   Organic oxygen compounds Organooxygen compounds
Glucose HMDB0000516 Organic oxygen compounds Organooxygen compounds
Glucose-1-P   Organic oxygen compounds Organooxygen compounds
Glyceraldehyde-3-P   Organic oxygen compounds Organooxygen compounds
Glyceric acid HMDB0160653 Organic oxygen compounds Organooxygen compounds
N-Acetyl-glucosamine   Organic oxygen compounds Organooxygen compounds
Ribose HMDB0000283 Organic oxygen compounds Organooxygen compounds
Ribose-5-P   Organic oxygen compounds Organooxygen compounds
Ribulose-5-P   Organic oxygen compounds Organooxygen compounds