Absolute Quantitation
Quantification of metabolites in folate cycle, methione cycle, choline metabolism, transsulfuration pathway and vitamin co-factors by LC-MS.
Price
Academic
$400 1st sample; $120 each additional sample
Industry
$800 1st sample; $240 each additional sample
Minimum Volume
Tissue: 25 mg
Cell: 5×10^6
Blood/plasma/serum: 100 µL
Urine: 100 µL
Plant: 100 mg
Feces: N/A
DBS: N/A
Instrument
UPLC-MRM/MS
Chemical Class
Folates,
SAM,
SAH,
dcSAM,
vitamin Bs,
reduced and oxidized glutathionine,
amino acids,
cysteine,
homocysteine,
cystathionine
Metabolic Pathways
Folate cycle,
methione cycle,
cysteine synthesis and metabolism,
choline and betaine degradation,
transsulfuration,
methylation pathway
Compounds
Fatty acids, acylcarnitines, short-chain fatt acids, medium chain fatty acids, long chain fatty acids, very long chain fatty acids, polyunsaturated fatty acids
| Name | HMDB ID | Super Class | Class |
| Acetyl choline | |||
| Dihydrofolate (DHF) | |||
| Glutathione oxidized (GSSG) | |||
| Glutathione reduced (GSH) | |||
| Tetrahydrofolate (THF) | |||
| Vitain B2 | |||
| dcSAM | Nucleosides, nucleotides, and analogues | 5′-deoxyribonucleosides | |
| SAH | Nucleosides, nucleotides, and analogues | 5′-deoxyribonucleosides | |
| SAM | Nucleosides, nucleotides, and analogues | 5′-deoxyribonucleosides | |
| Betaine | HMDB0000043 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Cystathionine | HMDB00099 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Cysteine | HMDB00574 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Cystine | HMDB0000192 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Folate | Organic acids and derivatives | Carboxylic acids and derivatives | |
| Glycine | HMDB0000123 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Homocysteine | HMDB00742 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Homocysteine | HMDB00742 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Homoserine | HMDB0000719 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Hypotaurine | Organic acids and derivatives | Sulfinic acids and derivatives | |
| Methionine | HMDB0000696 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Serine | HMDB0001264 | Organic acids and derivatives | Carboxylic acids and derivatives |
| Taurine | HMDB0000251 | Organic acids and derivatives | Organic sulfonic acids and derivatives |
| Choline | HMDB0000097 | Organic nitrogen compounds | Organonitrogen compounds |
| 5-Methyl THF | Organoheterocyclic compounds | Pteridines and derivatives | |
| Vitamin B12 | Organoheterocyclic compounds | Organoheterocyclic compounds | |
| Vitamin B6 | Organoheterocyclic compounds | Pyridines and derivatives |
