Absolute Quantitation
Detection and quantitation of isoprenyl phosphate intermediates, isoprenoids (ubiquinones, dolichols, vitamin K2 and squalene, etc.), cholesterol and other sterols by UPLC-MRM/MS and UPLC-FTMS.
Price
Academic
$400 1st sample; $180 each additional sample
Industry
$800 1st sample; $360 each additional sample
Minimum Volume
Tissue: 50 mg
Cell: 5×10^6
Blood/plasma/serum: 100 µL
Urine: N/A
Plant: N/A
Feces: 50 mg
DBS: 30 µL
Instrument
UPLC-MRM/MS
Chemical Class
Isoprenyl phosphates and pyrophospates,
HMG-CoA,
isoprenoids,
ubiquinones,
dolichols,
squalene,
oxidosqualene,
menaquinones (vitamin Ks),
sterols and cholesterol
Metabolic Pathways
Mevalonate pathway,
Isoprenoid synthesis
Cholesterol synthesis
Compounds
| Name | HMDB ID | Super Class | Class |
| 3-Hydroxy-3-methylglutaryl CoA (HMG-CoA) | |||
| Dolichols ( 13 to 21 isoprene units) | |||
| Isopentenyl pyrophosphate | |||
| Lathostenol | |||
| Menoquinones (vitamin Ks) | |||
| Mevalonic acid 5-phosphate | |||
| Ubiquinones —CoQ6, CoQ8, CoQ9, CoQ10, CoQ11, CoQ12 | |||
| 2,3-oxidosqualene | Lipids and lipid-like molecules | Prenol lipids | |
| 7-Dehydrocholesterol (provitamin D3) | Lipids and lipid-like molecules | Steroids and steroid derivatives | |
| 7-Dehydrodesmosterol | Lipids and lipid-like molecules | Steroids and steroid derivatives | |
| Acetoacetyl-CoA | Lipids and lipid-like molecules | Fatty Acyls | |
| Acetyl-CoA | Lipids and lipid-like molecules | Fatty Acyls | |
| Cholesterol | HMDB00067 | Lipids and lipid-like molecules | Steroids and steroid derivatives |
| Desmosterol | HMDB0002719 | Lipids and lipid-like molecules | Steroids and steroid derivatives |
| Dihydrolanosterol | Lipids and lipid-like molecules | Prenol lipids | |
| Dimethylallyl pyrophosphate | Lipids and lipid-like molecules | Prenol lipids | |
| Farnesyl pyrophosphate | Lipids and lipid-like molecules | Prenol lipids | |
| Geranyl pyrophosphate | Lipids and lipid-like molecules | Prenol lipids | |
| Geranylgeranyl pyrophosphate | Lipids and lipid-like molecules | Prenol lipids | |
| Lanosterol | Lipids and lipid-like molecules | Prenol lipids | |
| Mevalonic acid | HMDB0059629 | Lipids and lipid-like molecules | Fatty Acyls |
| Squalene | Lipids and lipid-like molecules | Prenol lipids | |
| Zymostenol | Lipids and lipid-like molecules | Steroids and steroid derivatives | |
| Zymosterol | Lipids and lipid-like molecules | Steroids and steroid derivatives | |
| Mevalonic acid 5-pyrophosphate | Organic oxygen compounds | Organic oxoanionic compounds | |
