Absolute and Relative Quantitation
Quantitation of purine/pyrimidine synthesis and salvage metabolites using a combined LC-MRM/MS and high-resolution LC-MS approach for absolute (with internal standards) and relative quantitation.
Price
Academic
$400 1st sample; $120 each additional sample
Industry
$800 1st sample; $240 each additional sample
Minimum Volume
Tissue: 20 mg
Cell: 5×10^6
Blood/plasma/serum: TBD
Urine: N/A
Plant: TBD
Instrument
UPLC-MRM/MS
Chemical Class
Nucleotide synthesis precursors,
intermediates and end products
Metabolic Pathways
Nucleotide synthesis,
IMP synthesis,
UMP synthesis
Compounds
| Name | HMDB ID | Super Class | Class |
| 5-aminoimidazole ribonucleotide (ribotide) (AIR) | |||
| 5-aminoimidazole-4-Carboxamide Riboside (AICAR) | |||
| 5-Formylamino-4-imidazolecarboxamide Ribonucleotide (FAICAR) | |||
| Carbamoyl aspartate | |||
| Carbamoyl phosphate | |||
| Carboxyaminoimidazole ribonucleotide (CAIR) | |||
| Dihydrorotate | |||
| Formylglycinamide Ribotide (FGAR) | |||
| Formylglycinamidine Ribotide (FGAM) | |||
| Glycinamide ribonucleotide (GAR) | |||
| N-Succinyl-5-aminoimidazole-4-carboxamide (SAICA) | |||
| N-Succinyl-5-aminoimidazole-4-carboxamide Riboside (SAICAR) | |||
| Orotidine-5P | |||
| Phosphoribosyl amine | |||
| IMP | HMDB00175 | Nucleosides, nucleotides, and analogues | Purine nucleotides |
| UMP | HMDB00288 | Nucleosides, nucleotides, and analogues | Pyrimidine nucleotides |
| Phosphoribosyl pyrophosphate (PRPP) | Organic oxygen compounds | Organooxygen compounds | |
| Ribose-5P | Organic oxygen compounds | Organooxygen compounds | |
| Orotate | Organoheterocyclic compounds | Diazines |
