Targeted Analysis of Polar Metabolites
Quantitative Metabolomics

Absolute Quantitation

A high sample throughput assay for identification and quantification of a wide range of polar metabolites ideal for large-scale studies, ionic metabolites, and low volume samples (<5 uL or < 5 mg dried weight).

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Price
Academic
$120
Industry
$200
Minimum Volume

Plasma/Serum: 50 uL
Cord blood: 50uL
DBS: 3.4 mm diameter punch
Urine: 10 uL
Sweat: 10 uL
Saliva: 10uL
Tissue (muscle, placenta, adipose): 5 mg dried mass
Cell cultures: 5 mg dried mass
Stool/Cecum: 5 mg dried mass

Instrument
UPLC-MRM/MS
Chemical Class

Acylcarnitines;
Amino acids;

Metabolic Pathways

amino acid metabolism,
lipid metabolism,
oxidation pathway,
thiol-redox pathway,
TCA cycle

Compounds

 

Name HMDB ID Super Class Class
(Iso)butyrylcarnitine (C4)      
3-Aminoisobutyric acid (BAIBA)      
3-Hydroxyanthranilic acid      
4-Aminohippuric acid      
5-Hydroxyindole acetic acid      
Acetyl-arginine      
Acetylcarnitine (C2)      
Argininosuccinate      
Arginosuccinate      
Asparic acid      
Asymmetric dimethylarginine (SDMA)      
Carboxy-ibuprofen      
Carnitine (C0)      
Cystine (disulfide)      
Cystine disulfide      
Decanoylcarnitine (C10)      
Dimethyl guanosine      
Dimethylglycine (DMG)      
Ethyl sulfate      
Ethylmalonic acid/Glutaric acid      
Fructose-1,6-bisphosphate (F1,6P)      
Fructose-6-phosphate (F6P)      
gamma-Aminobutyic acid (GABA)      
Glc-Hydroxylysine      
GlcGal-Hydroxylysine      
Glucose-1-phosphate (G1P)      
Glucuronic acid      
Glycerol phosphate      
Glytamyl-cysteine      
GSSG      
Guanidoacetic acid      
Hexanoylcarnitine (C6)      
Hydroxyphenylacetylglycine      
Hydroxyproline      
Hydroxyproline (HyPro)      
Imidazole propionate      
Indole-3-lactic acid      
Ketodeoxycholic acid      
L-Beta-Aspartyl-L-glycine      
Methylguanine      
Methyluric acid      
Monomethylarginine      
N-Acetylaspartic acid      
o/p/3-Hydroxyphenylacetic acid      
Octanoylcarnitine (C8)      
Phenyl alanine      
Phenylacetyl glutamine      
Phenylglyoxylic acid      
Pilocarpic acid      
Propionylcarnitine (C3)      
Pyridoxic acid      
Pyrocatechol sulfate      
Pyroglutamic acid      
Pyroglutamic acid (oxo-proline)      
Symmetric dimethylarginine (ADMA)      
Trigonelline      
N’-Nitrosoanabasine   Alkaloids and derivatives Alkaloids and derivatives
Pilocarpine   Alkaloids and derivatives Alkaloids and derivatives
3-Methoxytyramine   Benzenoids Benzenoids
Benzoic acid HMDB01870 Benzenoids Benzene and substituted derivatives
Dihydroxybenzoic acid   Benzenoids Benzenoids
Gentisuric acid   Benzenoids Benzenoids
Hippuric acid HMDB0062583 Benzenoids Benzene and substituted derivatives
Homogentisic acid HMDB0000130 Benzenoids Benzene and substituted derivatives
Methylparaben   Benzenoids Benzenoids
Phenylacetic acid   Benzenoids Benzenoids
Propylparaben   Benzenoids Benzenoids
Salicyluric acid HMDB00840 Benzenoids Benzene and substituted derivatives
Syringic acid   Benzenoids Benzene and substituted derivatives
Vanillic acid HMDB00484 Benzenoids Benzene and substituted derivatives
Vanillylmandelic acid HMDB00291 Benzenoids Phenols
Phosphate   Homogeneous non-metal compounds Homogeneous non-metal compounds
Phosphoric acid   Homogeneous non-metal compounds Homogeneous non-metal compounds
2-Ethylsuberic acid HMDB0059708 Lipids and lipid-like molecules Fatty Acyls
2-Hydroxyvaleric acid HMDB0001970 Lipids and lipid-like molecules Fatty Acyls
2-Isopropylmalic acid   Lipids and lipid-like molecules Lipids and lipid-like molecules
2-Methylglutaric acid   Lipids and lipid-like molecules Lipids and lipid-like molecules
3-Hydroxymethylglutaric acid HMDB0059737 Lipids and lipid-like molecules Fatty Acyls
3-Methylglutaconic acid HMDB0000522 Lipids and lipid-like molecules Fatty Acyls
Acetylcarnitine HMDB0000201 Lipids and lipid-like molecules Fatty Acyls
Azelaic acid HMDB00784 Lipids and lipid-like molecules Fatty Acyls
Butyric acid   Lipids and lipid-like molecules Fatty Acyls
Butyryl-L-carnitine   Lipids and lipid-like molecules Lipids and lipid-like molecules
Capric acid HMDB0000513 Lipids and lipid-like molecules Fatty Acyls
Caprylic acid HMDB0062511 Lipids and lipid-like molecules Fatty Acyls
Cholic acid HMDB0000619 Lipids and lipid-like molecules Steroids and steroid derivatives
Citraconic acid   Lipids and lipid-like molecules Lipids and lipid-like molecules
Citramalic acid HMDB0176421 Lipids and lipid-like molecules Fatty Acyls
Deoxycarnitine   Lipids and lipid-like molecules Lipids and lipid-like molecules
Deoxycholic acid HMDB0000626 Lipids and lipid-like molecules Steroids and steroid derivatives
Glycerophosphorylcholine   Lipids and lipid-like molecules Lipids and lipid-like molecules
Isovaleric acid   Lipids and lipid-like molecules Fatty Acyls
Lauric acid HMDB0000638 Lipids and lipid-like molecules Fatty Acyls
Malonyl-L-carnitine   Lipids and lipid-like molecules Lipids and lipid-like molecules
Methylsuccinic acid HMDB0029127 Lipids and lipid-like molecules Fatty Acyls
Pimelic acid   Lipids and lipid-like molecules Fatty Acyls
Propionyl-L-carnitine   Lipids and lipid-like molecules Lipids and lipid-like molecules
Suberic acid HMDB00893 Lipids and lipid-like molecules Fatty Acyls
Taurocholic Acid HMDB0000036 Lipids and lipid-like molecules Steroids and steroid derivatives
Taurodeoxycholic Acid HMDB0000896 Lipids and lipid-like molecules Steroids and steroid derivatives
Tiglyl-L-carnitine   Lipids and lipid-like molecules Lipids and lipid-like molecules
1-Methylguanosine   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
ADP HMDB01341 Nucleosides, nucleotides, and analogues Purine nucleotides
AMP HMDB00045 Nucleosides, nucleotides, and analogues Purine nucleotides
ATP HMDB00538 Nucleosides, nucleotides, and analogues Purine nucleotides
cAMP   Nucleosides, nucleotides, and analogues Purine nucleotides
GTP HMDB01273 Nucleosides, nucleotides, and analogues Purine nucleotides
IMP HMDB00175 Nucleosides, nucleotides, and analogues Purine nucleotides
Methylcytidine   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
NAD+   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
NADP+   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
Pseudouridine   Nucleosides, nucleotides, and analogues Nucleosides, nucleotides, and analogues
UTP   Nucleosides, nucleotides, and analogues Pyrimidine nucleotides
2-Aminooctanoic acid HMDB0000991 Organic acids and derivatives Carboxylic acids and derivatives
2-Furoylglycine HMDB00439 Organic acids and derivatives Carboxylic acids and derivatives
2-Hydroxy-5-oxoproline   Organic acids and derivatives Organic acids and derivatives
2-Hydroxybutyric acid HMDB0030097 Organic acids and derivatives Hydroxy acids and derivatives
2-Methylbutyrylglycine HMDB0000339 Organic acids and derivatives Carboxylic acids and derivatives
3-Hydroxybutyric acid HMDB0000766 Organic acids and derivatives Hydroxy acids and derivatives
3-Methyl-2-oxovaleric acid HMDB0000491 Organic acids and derivatives Keto acids and derivatives
3-Methylhistidine HMDB0155761 Organic acids and derivatives Carboxylic acids and derivatives
4-Guanidinobutanoic acid   Organic acids and derivatives Organic acids and derivatives
5-Aminolevulinic acid HMDB0001149 Organic acids and derivatives Carboxylic acids and derivatives
5-Aminopentanoic acid HMDB0003355 Organic acids and derivatives Carboxylic acids and derivatives
Acesulfame K   Organic acids and derivatives Organic acids and derivatives
Acetaminophen sulfate   Organic acids and derivatives Organic sulfuric acids and derivatives
Acetate HMDB00042 Organic acids and derivatives Carboxylic acids and derivatives
Acetoacetic acid HMDB0000060 Organic acids and derivatives Keto acids and derivatives
Acetylcarnosine HMDB0012881 Organic acids and derivatives Peptidomimetics
Alanine HMDB0061370 Organic acids and derivatives Carboxylic acids and derivatives
Alpha-aminoadipic acid   Organic acids and derivatives Organic acids and derivatives
Alpha-ketoisovaleric acid   Organic acids and derivatives Organic acids and derivatives
Aminoadipic acid HMDB0062113 Organic acids and derivatives Carboxylic acids and derivatives
Arginine HMDB0003416 Organic acids and derivatives Carboxylic acids and derivatives
Asparagine HMDB0000168 Organic acids and derivatives Carboxylic acids and derivatives
Aspartic acid HMDB00191 Organic acids and derivatives Carboxylic acids and derivatives
Betaine HMDB0000043 Organic acids and derivatives Carboxylic acids and derivatives
Carnosine HMDB00033 Organic acids and derivatives Peptidomimetics
Cis-Aconitic acid   Organic acids and derivatives Organic acids and derivatives
Citric acid HMDB0000094 Organic acids and derivatives Carboxylic acids and derivatives
Citrulline HMDB0000904 Organic acids and derivatives Carboxylic acids and derivatives
Creatine HMDB0000064 Organic acids and derivatives Carboxylic acids and derivatives
Creatinine HMDB0000562 Organic acids and derivatives Carboxylic acids and derivatives
Cystathionine HMDB00099 Organic acids and derivatives Carboxylic acids and derivatives
Cysteine HMDB00574 Organic acids and derivatives Carboxylic acids and derivatives
Cysteineglutathione disulfide   Organic acids and derivatives Organic acids and derivatives
Cysteinylglycine   Organic acids and derivatives Organic acids and derivatives
Cysteinylglycine disulfide HMDB0000709 Organic acids and derivatives Carboxylic acids and derivatives
Cystine HMDB0000192 Organic acids and derivatives Carboxylic acids and derivatives
Fumaric acid HMDB00134 Organic acids and derivatives Carboxylic acids and derivatives
GABA   Organic acids and derivatives Organic acids and derivatives
Glutaconic acid   Organic acids and derivatives Organic acids and derivatives
Glutamate HMDB0060475 Organic acids and derivatives Carboxylic acids and derivatives
Glutamic acid HMDB0000148 Organic acids and derivatives Carboxylic acids and derivatives
Glutamine HMDB0133927 Organic acids and derivatives Carboxylic acids and derivatives
Glutaric acid HMDB0034365 Organic acids and derivatives Carboxylic acids and derivatives
Glycine HMDB0000123 Organic acids and derivatives Carboxylic acids and derivatives
Glycolic acid HMDB0000115 Organic acids and derivatives Hydroxy acids and derivatives
Glycylhistidine   Organic acids and derivatives Organic acids and derivatives
Glycylproline HMDB0000721 Organic acids and derivatives Carboxylic acids and derivatives
GSH   Organic acids and derivatives Organic acids and derivatives
Guanidinosuccinic acid HMDB0003157 Organic acids and derivatives Carboxylic acids and derivatives
Hexanoylglycine HMDB00701 Organic acids and derivatives Carboxylic acids and derivatives
Histidine HMDB0154888 Organic acids and derivatives Carboxylic acids and derivatives
Homoarginine   Organic acids and derivatives Carboxylic acids and derivatives
Homocitrulline HMDB00679 Organic acids and derivatives Carboxylic acids and derivatives
Hydroxyethyl glycine HMDB0061148 Organic acids and derivatives Carboxylic acids and derivatives
Hydroxylysine   Organic acids and derivatives Organic acids and derivatives
Hypotaurine   Organic acids and derivatives Sulfinic acids and derivatives
Iminodiacetic acid   Organic acids and derivatives Organic acids and derivatives
Indoleacetyl glutamine   Organic acids and derivatives Carboxylic acids and derivatives
Indolylacryloylglycine   Organic acids and derivatives Organic acids and derivatives
indoxyl sulfate HMDB0186746 Organic acids and derivatives Organic sulfuric acids and derivatives
Isoleucine HMDB0000172 Organic acids and derivatives Carboxylic acids and derivatives
Lactic acid HMDB0041561 Organic acids and derivatives Hydroxy acids and derivatives
Lauryl sulfate   Organic acids and derivatives Organic acids and derivatives
Leucine HMDB0000687 Organic acids and derivatives Carboxylic acids and derivatives
Levulinic acid HMDB0029696 Organic acids and derivatives Keto acids and derivatives
Lysine HMDB0000182 Organic acids and derivatives Carboxylic acids and derivatives
Malic acid HMDB0167333 Organic acids and derivatives Hydroxy acids and derivatives
Methionine HMDB0000696 Organic acids and derivatives Carboxylic acids and derivatives
Methionine Sulfoxide HMDB0002005 Organic acids and derivatives Carboxylic acids and derivatives
N-Acetylputrescine   Organic acids and derivatives Carboximidic acids and derivatives
N-Butyrylglycine HMDB0000808 Organic acids and derivatives Carboxylic acids and derivatives
Nicotinuric acid   Organic acids and derivatives Organic acids and derivatives
Ornithine HMDB0062620 Organic acids and derivatives Carboxylic acids and derivatives
Oxo-proline   Organic acids and derivatives Organic acids and derivatives
Oxoglutarate HMDB00208 Organic acids and derivatives Keto acids and derivatives
Oxoglutaric acid HMDB0000346 Organic acids and derivatives Keto acids and derivatives
p-Cresol sulfate HMDB0133325 Organic acids and derivatives Organic sulfuric acids and derivatives
Phenyl sulfate   Organic acids and derivatives Organic acids and derivatives
Phenylacetylglutamine HMDB0006344 Organic acids and derivatives Carboxylic acids and derivatives
Phenylacetylglycine HMDB0000821 Organic acids and derivatives Carboxylic acids and derivatives
Phenylalanine HMDB0159744 Organic acids and derivatives Carboxylic acids and derivatives
Phenylpropionylglycine   Organic acids and derivatives Organic acids and derivatives
Phenylsulfate   Organic acids and derivatives Organic sulfuric acids and derivatives
Phosphocreatine   Organic acids and derivatives Carboxylic acids and derivatives
Pipecolate HMDB00070 Organic acids and derivatives Carboxylic acids and derivatives
Pipecolic acid HMDB0062767 Organic acids and derivatives Carboxylic acids and derivatives
Proline HMDB0000162 Organic acids and derivatives Carboxylic acids and derivatives
Proline betaine HMDB0004827 Organic acids and derivatives Carboxylic acids and derivatives
Prolylglycine HMDB0011178 Organic acids and derivatives Carboxylic acids and derivatives
Propenoyl-L-carnitine   Organic acids and derivatives Organic acids and derivatives
Propionate HMDB00237 Organic acids and derivatives Carboxylic acids and derivatives
Propionylglycine HMDB0000783 Organic acids and derivatives Carboxylic acids and derivatives
Pyridylacetylglycine   Organic acids and derivatives Organic acids and derivatives
Pyroglutamic acid HMDB0000267 Organic acids and derivatives Carboxylic acids and derivatives
Pyruvic acid HMDB0141776 Organic acids and derivatives Keto acids and derivatives
Serine HMDB0001264 Organic acids and derivatives Carboxylic acids and derivatives
Succinic acid HMDB0000254 Organic acids and derivatives Carboxylic acids and derivatives
Succinylacetone HMDB00635 Organic acids and derivatives Keto acids and derivatives
Taurine HMDB0000251 Organic acids and derivatives Organic sulfonic acids and derivatives
Theanine HMDB0062764 Organic acids and derivatives Carboxylic acids and derivatives
Threonine HMDB0000167 Organic acids and derivatives Carboxylic acids and derivatives
Tiglylglycine HMDB0002393 Organic acids and derivatives Carboxylic acids and derivatives
Trimethyllysine   Organic acids and derivatives Organic acids and derivatives
Tryptophan betaine   Organic acids and derivatives Organic acids and derivatives
Tyrosine HMDB0000158 Organic acids and derivatives Carboxylic acids and derivatives
Urea HMDB00294 Organic acids and derivatives Organic carbonic acids and derivatives
Valine HMDB0000883 Organic acids and derivatives Carboxylic acids and derivatives
Valylvaline   Organic acids and derivatives Organic acids and derivatives
1-Phenylethylamine HMDB0002017 Organic nitrogen compounds Organonitrogen compounds
Acetylcholine HMDB0000895 Organic nitrogen compounds Organonitrogen compounds
Cadaverine HMDB02322 Organic nitrogen compounds Organonitrogen compounds
Carnitine HMDB0062496 Organic nitrogen compounds Organonitrogen compounds
Choline HMDB0000097 Organic nitrogen compounds Organonitrogen compounds
Phosphorylcholine HMDB0001565 Organic nitrogen compounds Organonitrogen compounds
Putrescine HMDB01414 Organic nitrogen compounds Organonitrogen compounds
Spermidine   Organic nitrogen compounds Organonitrogen compounds
Spermine   Organic nitrogen compounds Organonitrogen compounds
Trimethylamine N-oxide HMDB0000925 Organic nitrogen compounds Organonitrogen compounds
2,3-Dehydro-2-deoxy-N-acetylneuraminic acid   Organic oxygen compounds Organic oxygen compounds
Acetaminophen glucuronide   Organic oxygen compounds Organooxygen compounds
Acetylneuraminic acid   Organic oxygen compounds Organic oxygen compounds
CTP   Organic oxygen compounds Organooxygen compounds
Ethyl glucuronide   Organic oxygen compounds Organooxygen compounds
Glucaric acid   Organic oxygen compounds Organooxygen compounds
Gluconic acid   Organic oxygen compounds Organooxygen compounds
Glucose HMDB0000516 Organic oxygen compounds Organooxygen compounds
Glucose-6-phosphate (G6P)   Organic oxygen compounds Organooxygen compounds
Glyceraldehyde 3-phosphate   Organic oxygen compounds Organooxygen compounds
Kynurenine HMDB0000684 Organic oxygen compounds Organooxygen compounds
N-Acetyl-9-O-acetylneuraminic acid   Organic oxygen compounds Organic oxygen compounds
N-acetylneuraminic acid HMDB0000230 Organic oxygen compounds Organooxygen compounds
N-Glycolylneuraminic acid   Organic oxygen compounds Organic oxygen compounds
p-Cresol glucuronide   Organic oxygen compounds Organic oxygen compounds
Pantothenic acid HMDB0062717 Organic oxygen compounds Organooxygen compounds
Quinic acid HMDB0172071 Organic oxygen compounds Organooxygen compounds
Tartaric acid HMDB0000346 Organic oxygen compounds Organooxygen compounds
Threonic acid HMDB0137260 Organic oxygen compounds Organooxygen compounds
1-Methylnicotinamide HMDB0000699 Organoheterocyclic compounds Pyridines and derivatives
1-Methyluric acid HMDB0001882 Organoheterocyclic compounds Imidazopyrimidines
1,3-Dimethyluric acid HMDB0030801 Organoheterocyclic compounds Imidazopyrimidines
3-Methyladenine   Organoheterocyclic compounds Organoheterocyclic compounds
5-Acetylamino-6-formylamino-3-methyluracil   Organoheterocyclic compounds Organoheterocyclic compounds
7-Methylxanthine HMDB0001991 Organoheterocyclic compounds Imidazopyrimidines
Adenine HMDB00034 Organoheterocyclic compounds Imidazopyrimidines
Ascorbic acid HMDB0000044 Organoheterocyclic compounds Dihydrofurans
Caffeine HMDB0001847 Organoheterocyclic compounds Imidazopyrimidines
Dihydrothymine   Organoheterocyclic compounds Organoheterocyclic compounds
Guanine   Organoheterocyclic compounds Imidazopyrimidines
Hydroxycotinine   Organoheterocyclic compounds Organoheterocyclic compounds
Hypoxanthine HMDB0000157 Organoheterocyclic compounds Imidazopyrimidines
Indole-3-acetamide   Organoheterocyclic compounds Organoheterocyclic compounds
Indole-3-carboxaldehyde   Organoheterocyclic compounds Organoheterocyclic compounds
Indoleacetic acid HMDB0000197 Organoheterocyclic compounds Indoles and derivatives
Kynurenic Acid HMDB00715 Organoheterocyclic compounds Quinolines and derivatives
Orotic acid   Organoheterocyclic compounds Diazines
Oxypurinol HMDB0000786 Organoheterocyclic compounds Imidazopyrimidines
Quinolinic acid HMDB00232 Organoheterocyclic compounds Pyridines and derivatives
Saccharin   Organoheterocyclic compounds Organoheterocyclic compounds
Thymine HMDB0010337 Organoheterocyclic compounds Diazines
Trans-urocanate   Organoheterocyclic compounds Organoheterocyclic compounds
Trans-urocanic acid   Organoheterocyclic compounds Organoheterocyclic compounds
Tryptophan HMDB0030396 Organoheterocyclic compounds Indoles and derivatives
Uracil HMDB0000300 Organoheterocyclic compounds Diazines
Uric acid HMDB0000289 Organoheterocyclic compounds Imidazopyrimidines
Urocanic acid HMDB0062562 Organoheterocyclic compounds Azoles
Xanthine HMDB0000292 Organoheterocyclic compounds Imidazopyrimidines
Cinnamic acid   Phenylpropanoids and polyketides Phenylpropanoids and polyketides
Ferulic acid HMDB0000954 Phenylpropanoids and polyketides Cinnamic acids and derivatives